Substantial reduction in cycle time required to identify and isolate targets
High-speed sorting using FACS enables a reduction in the amount of time to sort from 3 or 4 months to 25 days—an approximate 75% improvement.
Significant increase in yield of positives identified and cloned in each cycle
DiSH technology deposits target hybridomas in single cell wells, avoiding the loss of high quality prospects due to overgrowth from competing cells, resulting in nearly a ten-fold increase in positives versus conventional methods.
Dramatic increase in the number of stable positives isolated in each cycle
Using traditional methods, many positive clones fail to remain viable due to issues with chromosome structure. Comparatively, 91% of positive hybridomas remained stable through the cloning process using DiSH versus 23% of positives when using conventional hybridoma technology.
Cleanly aligns with current hybridoma screening and production methods
Unlike selected competitive technologies that require specialized laboratory training, workflow and infrastructure, the DiSH protocol closely parallels the industry's dominant hybridoma development methodology, making adoption simple and straightforward.